PDBe 4d1n

X-ray diffraction
2.03Å resolution

Structure of human nNOS heme domain with L-Arg bound

Released:
Source organism: Homo sapiens
Primary publication:
Structures of human constitutive nitric oxide synthases.
Acta Crystallogr. D Biol. Crystallogr. 70 2667-74 (2014)
PMID: 25286850

Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase, brain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 420 amino acids
Theoretical weight: 48.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29475 (Residues: 302-721; Coverage: 29%)
Gene name: NOS1
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 174.923Å b: 84.664Å c: 166.745Å
α: 90° β: 90.94° γ: 90°
R-values:
R R work R free
0.179 0.177 0.209
Expression system: Escherichia coli BL21(DE3)