PDBe 4cmq

X-ray diffraction
3.09Å resolution

Crystal structure of Mn-bound S.pyogenes Cas9

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas9/Csn1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1372 amino acids
Theoretical weight: 158.99 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21212
Unit cell:
a: 159.88Å b: 210.12Å c: 90.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.249 0.277
Expression system: Escherichia coli BL21(DE3)