› 4ckj

X-ray diffraction
1.65Å resolution

Crystal structure of RET tyrosine kinase domain bound to adenosine

Released:
DOI: 10.2210/pdb4ckj/pdb
PDB entry 4ckj coloured by chain, front view.
PDB entry 4ckj coloured by chain, side view.
PDB entry 4ckj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Oncogenic RET kinase domain mutations perturb the autophosphorylation trajectory by enhancing substrate presentation in trans.
Plaza-Menacho I, Barnouin K, Goodman K, Martínez-Torres RJ, Borg A, Murray-Rust J, Mouilleron S, Knowles P, McDonald NQ
Mol Cell 53 738-51 (2014)
PMID: 24560924

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139854 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble RET kinase fragment Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 35.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07949 (Residues: 705-1013; Coverage: 29%)
Gene names: CDHF12, CDHR16, PTC, RET
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADN 1 x ADN
Ligand FMT 16 x FMT
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 73.19Å b: 69.09Å c: 78.64Å
α: 90° β: 101.76° γ: 90°
R-values:
R R work R free
0.155 0.153 0.181
Expression system: Spodoptera frugiperda

Quick links

Citations

12 review citations

Mechanisms of RET signaling in cancer: current and future implications for targeted therapy.
Plaza-Menacho et al. (2014)
11 more

8 mentions without citation

Oncogenic RET kinase domain mutations perturb the autophosphorylation trajectory by enhancing substrate presentation in trans.
Plaza-Menacho et al. (2014)
7 more