PDBe 4ch2

X-ray diffraction
1.6Å resolution

Low-salt crystal structure of a thrombin-GpIbalpha peptide complex

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombin light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
Thrombin heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 259 amino acids
Theoretical weight: 29.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Glycocalicin Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 14 amino acids
Theoretical weight: 1.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P07359 (Residues: 287-300; Coverage: 2%)
Gene name: GP1BA

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 149.57Å b: 50.56Å c: 76.28Å
α: 90° β: 97.15° γ: 90°
R-values:
R R work R free
0.156 0.155 0.187
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided