PDBe 4c78

X-ray diffraction
2Å resolution

Complex of human Sirt3 with Bromo-Resveratrol and ACS2 peptide

Released:

Function and Biology Details

Reaction catalysed:
ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacetylase sirtuin-3, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 31.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NTG7 (Residues: 116-399; Coverage: 71%)
Gene names: SIR2L3, SIRT3
Sequence domains: Sir2 family
Acetyl-coenzyme A synthetase 2-like, mitochondrial Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.25 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NUB1 (Residues: 638-647; Coverage: 2%)
Gene names: ACAS2L, ACSS1, KIAA1846

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Unit cell:
a: 34.7Å b: 52.6Å c: 159.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.241
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided