PDBe 4c6o

X-ray diffraction
1.65Å resolution

Crystal structure of the dihydroorotase domain of human CAD C1613S mutant in apo-form at pH 6.0

Released:

Function and Biology Details

Reactions catalysed:
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. 
(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate. 
2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. 
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CAD protein Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 42.9 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P27708 (Residues: 1456-1846; Coverage: 18%)
Gene name: CAD
Sequence domains: Amidohydrolase family

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 82.12Å b: 159.42Å c: 61.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 0.115 0.157
Expression system: Homo sapiens