PDBe 4c11

X-ray diffraction
2.6Å resolution

Dengue virus RNA dependent RNA polymerase with residues from the NS5 linker region

Released:

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)- (purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)- methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)- [mRNA]. 
ATP + H(2)O = ADP + phosphate. 
NTP + H(2)O = NDP + phosphate. 
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala. 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). 
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-directed RNA polymerase NS5 Chain: A
Molecule details ›
Chain: A
Length: 638 amino acids
Theoretical weight: 73.96 KDa
Source organism: Dengue virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6YMS4 (Residues: 2753-3390; Coverage: 19%)
Gene name: pol
RNA-directed RNA polymerase NS5 Chain: B
Molecule details ›
Chain: B
Length: 638 amino acids
Theoretical weight: 73.99 KDa
Source organism: Dengue virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6YMS4 (Residues: 2753-3390; Coverage: 19%)
Gene name: pol

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FRE
Spacegroup: P21212
Unit cell:
a: 122.99Å b: 136.05Å c: 103.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.245
Expression system: Escherichia coli