PDBe 4bvu

X-ray diffraction
2.7Å resolution

Structure of Shigella effector OspG in complex with host UbcH5c- Ubiquitin conjugate

Released:

Function and Biology Details

Reactions catalysed:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
(1a) S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-monoubiquitinyl-[(E3-independent) ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin Chain: C
Molecule details ›
Chain: C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Protein kinase OspG Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 24.39 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99PZ6 (Residues: 1-196; Coverage: 100%)
Gene names: CP0227, SFLP087, ospG, pWR501_0237
Structure domains:
Ubiquitin-conjugating enzyme E2 D3 Chain: B
Molecule details ›
Chain: B
Length: 147 amino acids
Theoretical weight: 16.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61077 (Residues: 1-147; Coverage: 100%)
Gene names: UBC5C, UBCH5C, UBE2D3
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P6322
Unit cell:
a: 183.873Å b: 183.873Å c: 63.973Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.244 0.291
Expression system: Escherichia coli BL21