PDBe 4b86

X-ray diffraction
3.5Å resolution

Crystal structure of the MSL1-MSL2 complex (3.5A)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Male-specific lethal 1 homolog Chains: A, B, E, F, I, J
Molecule details ›
Chains: A, B, E, F, I, J
Length: 59 amino acids
Theoretical weight: 6.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q68DK7 (Residues: 212-267; Coverage: 9%)
Gene names: MSL1, MSL1L1
Sequence domains: Dimerisation domain of Male-specific-Lethal 1
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
E3 ubiquitin-protein ligase MSL2 Chains: C, D, G, H, K, L
Molecule details ›
Chains: C, D, G, H, K, L
Length: 116 amino acids
Theoretical weight: 13.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HCI7 (Residues: 1-116; Coverage: 20%)
Gene names: KIAA1585, MSL2, MSL2L1, RNF184
Sequence domains: zinc RING finger of MSL2
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 104.65Å b: 182.21Å c: 89.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.256 0.297
Expression system: Escherichia coli BL21(DE3)