PDBe 4b2d

X-ray diffraction
2.3Å resolution

human PKM2 with L-serine and FBP bound.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pyruvate kinase PKM Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 548 amino acids
Theoretical weight: 59.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14618 (Residues: 2-531; Coverage: 100%)
  • Best match: P14618-3 (Residues: 68-516)
Gene names: OIP3, PK2, PK3, PKM, PKM2
Sequence domains:
Structure domains:
Pyruvate kinase PKM Chain: D
Molecule details ›
Chain: D
Length: 548 amino acids
Theoretical weight: 59.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14618 (Residues: 2-531; Coverage: 100%)
  • Best match: P14618-3 (Residues: 68-516)
Gene names: OIP3, PK2, PK3, PKM, PKM2
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 80.611Å b: 151.098Å c: 91.791Å
α: 90° β: 102.01° γ: 90°
R-values:
R R work R free
0.176 0.174 0.226
Expression system: Escherichia coli BL21(DE3)