PDBe 4b18

X-ray diffraction
2.52Å resolution

The crystal structure of human Importin alpha 5 with TERT NLS peptide

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-5, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 49.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P52294 (Residues: 66-512; Coverage: 83%)
Gene names: KPNA1, RCH2
Sequence domains: Armadillo/beta-catenin-like repeat
Structure domains: Leucine-rich Repeat Variant
Telomerase reverse transcriptase Chain: B
Molecule details ›
Chain: B
Length: 20 amino acids
Theoretical weight: 2.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O14746 (Residues: 222-240; Coverage: 2%)
Gene names: EST2, TCS1, TERT, TRT

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 159.723Å b: 59.916Å c: 69.331Å
α: 90° β: 96.29° γ: 90°
R-values:
R R work R free
0.201 0.198 0.253
Expression systems:
  • Escherichia coli BL21
  • Not provided