PDBe 4awn

X-ray diffraction
1.95Å resolution

Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyribonuclease-1 Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.28 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P24855 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1, DRNI
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 41.96Å b: 58.66Å c: 96.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.166 0.218
Expression system: Cricetulus griseus