PDBe 4au2

X-ray diffraction
2.3Å resolution

Crystal Structure of a Hsp47-collagen complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serpin peptidase inhibitor, clade H (Heat shock protein 47), member 1, (Collagen binding protein 1) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 392 amino acids
Theoretical weight: 44.28 KDa
Source organism: Canis lupus familiaris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C7C419 (Residues: 36-418; Coverage: 96%)
Gene name: SERPINH1
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:
Collagen alpha-1(XXVI) chain Chains: E, F, G, H, I, J
Molecule details ›
Chains: E, F, G, H, I, J
Length: 16 amino acids
Theoretical weight: 1.3 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96A83 (Residues: 312-325; Coverage: 3%)
Gene names: COL26A1, EMID2, EMU2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 46.498Å b: 92.784Å c: 100.458Å
α: 95.24° β: 98.95° γ: 96.73°
R-values:
R R work R free
0.204 0.202 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided