› 4arz

X-ray diffraction
3.1Å resolution

The crystal structure of Gtr1p-Gtr2p complexed with GTP-GDP

Released:
DOI: 10.2210/pdb4arz/pdb
PDB entry 4arz coloured by chain, front view.
PDB entry 4arz coloured by chain, side view.
PDB entry 4arz coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the Gtr1p(GTP)-Gtr2p(GDP) protein complex reveals large structural rearrangements triggered by GTP-to-GDP conversion.
Jeong JH, Lee KH, Kim YM, Kim DH, Oh BH, Kim YG
J Biol Chem 287 29648-53 (2012)
PMID: 22807443

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156747 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding protein GTR1 Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 35.89 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00582 (Residues: 1-310; Coverage: 100%)
Gene names: GTR1, YM7056.05, YML121W
Sequence domains: Gtr1/RagA G protein conserved region
Structure domains:
GTP-binding protein GTR2 Chain: B
Molecule details ›
Chain: B
Length: 341 amino acids
Theoretical weight: 38.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P53290 (Residues: 1-341; Coverage: 100%)
Gene names: GTR2, YGR163W
Sequence domains: Gtr1/RagA G protein conserved region
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: C2221
Unit cell:
a: 90.712Å b: 148.897Å c: 117.759Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.272
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Amino acid signalling upstream of mTOR.
Jewell et al. (2013)
11 more

11 mentions without citation

Discovery of new Longin and Roadblock domains that form platforms for small GTPases in Ragulator and TRAPP-II.
Levine et al. (2013)
10 more