PDBe 4apc

X-ray diffraction
2.1Å resolution

Crystal Structure of Human NIMA-related Kinase 1 (NEK1)

Released:
Source organism: Homo sapiens
Entry authors: Elkins JM, Hanchuk TDM, Lovato DV, Basei FL, Meirelles GV, Kobarg J, Szklarz M, Vollmar M, Mahajan P, Rellos P, Zhang Y, Krojer T, Pike ACW, Bountra C, Arrowsmith C, Edwards A, Knapp S

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase Nek1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 39.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96PY6 (Residues: 1-328; Coverage: 26%)
Gene names: KIAA1901, NEK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2221
Unit cell:
a: 89.91Å b: 92.81Å c: 163.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.248
Expression system: Escherichia coli BL21(DE3)