› 4ak2

X-ray diffraction
1.35Å resolution

Structure of BT4661, a SusE-like surface located polysaccharide binding protein from the Bacteroides thetaiotaomicron heparin utilisation locus

Released:
DOI: 10.2210/pdb4ak2/pdb
PDB entry 4ak2 coloured by chain, front view.
PDB entry 4ak2 coloured by chain, side view.
PDB entry 4ak2 coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
How members of the human gut microbiota overcome the sulfation problem posed by glycosaminoglycans.
Cartmell A, Lowe EC, Baslé A, Firbank SJ, Ndeh DA, Murray H, Terrapon N, Lombard V, Henrissat B, Turnbull JE, Czjzek M, Gilbert HJ, Bolam DN
Proc Natl Acad Sci U S A 114 7037-7042 (2017)
PMID: 28630303

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
DUF4958 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 288 amino acids
Theoretical weight: 32.01 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q89YS0 (Residues: 448-726; Coverage: 40%)
Gene name: BT_4661
Sequence domains: Surface glycan binding protein BT4661-like
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: SGN, IDS
Carbohydrate polymer
1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 52.6Å b: 68.15Å c: 85.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.191
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

The Pros and Cons of Using Algal Polysaccharides as Prebiotics.
Gotteland et al. (2020)
12 more

5 mentions without citation

Docking server for the identification of heparin binding sites on proteins.
Mottarella et al. (2014)
4 more