› 4aie

X-ray diffraction
2.05Å resolution

Structure of glucan-1,6-alpha-glucosidase from Lactobacillus acidophilus NCFM

Released:
DOI: 10.2210/pdb4aie/pdb
PDB entry 4aie coloured by chain, front view.
PDB entry 4aie coloured by chain, side view.
PDB entry 4aie coloured by chain, top view.
Source organism: Lactobacillus acidophilus NCFM
Primary publication:
Enzymology and structure of the GH13_31 glucan 1,6-α-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM.
Møller MS, Fredslund F, Majumder A, Nakai H, Poulsen JC, Lo Leggio L, Svensson B, Abou Hachem M
J Bacteriol 194 4249-59 (2012)
PMID: 22685275

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in (1->6)-alpha-D-glucans and derived oligosaccharides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177117 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 549 amino acids
Theoretical weight: 64.31 KDa
Source organism: Lactobacillus acidophilus NCFM
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5FMB7 (Residues: 2-539; Coverage: 100%)
Gene name: LBA0264
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MES 3 x MES
Ligand CA 1 x CA
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P21212
Unit cell:
a: 55.83Å b: 107.25Å c: 103.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.137 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

α-Amylase: an enzyme specificity found in various families of glycoside hydrolases.
Janeček et al. (2014)
7 more

3 mentions without citation

Enzymology and structure of the GH13_31 glucan 1,6-α-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM.
Møller et al. (2012)
2 more