› 3zyz

X-ray diffraction
2.1Å resolution

Crystal structure of a glycoside hydrolase family 3 beta-glucosidase, Bgl1 from Hypocrea jecorina at 2.1A resolution.

Released:
DOI: 10.2210/pdb3zyz/pdb
PDB entry 3zyz coloured by chain, front view.
PDB entry 3zyz coloured by chain, side view.
PDB entry 3zyz coloured by chain, top view.
Source organism: Trichoderma reesei
Primary publication:
Biochemical characterization and crystal structures of a fungal family 3 β-glucosidase, Cel3A from Hypocrea jecorina.
Karkehabadi S, Helmich KE, Kaper T, Hansson H, Mikkelsen NE, Gudmundsson M, Piens K, Fujdala M, Banerjee G, Scott-Craig JS, Walton JD, Phillips GN, Sandgren M
J Biol Chem 289 31624-37 (2014)
PMID: 25164811

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 713 amino acids
Theoretical weight: 75.4 KDa
Source organism: Trichoderma reesei
UniProt:
  • Canonical: Q12715 (Residues: 32-744; Coverage: 96%)
Gene name: bgl1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NAG 2 x NAG
Ligand BGC 1 x BGC
Ligand GOL 1 x GOL
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 55.065Å b: 82.286Å c: 136.811Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.172 0.222

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Citations

3 review citations

Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update for 2013-2014.
Harvey DJ. (2018)
2 more

19 mentions without citation

Biochemical characterization and crystal structures of a fungal family 3 β-glucosidase, Cel3A from Hypocrea jecorina.
Karkehabadi et al. (2014)
18 more