PDBe 3zx6

X-ray diffraction
2.65Å resolution

Structure of Hamp(AF1503)-Tsr fusion - Hamp (A291V) mutant

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HAMP domain-containing protein; Methyl-accepting chemotaxis protein I Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 36.14 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02942 (Residues: 264-518, 519-551; Coverage: 52%)
  • Canonical: O28769 (Residues: 278-326; Coverage: 15%)
  • nullnull
Gene names: AF_1503, JW4318, b4355, cheD, tsr
Sequence domains: Methyl-accepting chemotaxis protein (MCP) signalling domain
Structure domains: Methyl-accepting chemotaxis protein

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 112.22Å b: 80.02Å c: 139.17Å
α: 90° β: 110.78° γ: 90°
R-values:
R R work R free
0.231 0.229 0.283
Expression system: Escherichia coli BL21(DE3)