› 3zuc

X-ray diffraction
1Å resolution

Structure of CBM3b of major scaffoldin subunit ScaA from Acetivibrio cellulolyticus determined from the crystals grown in the presence of Nickel

Released:
DOI: 10.2210/pdb3zuc/pdb
PDB entry 3zuc coloured by chain, front view.
PDB entry 3zuc coloured by chain, side view.
PDB entry 3zuc coloured by chain, top view.
Source organism: Acetivibrio cellulolyticus
Primary publication:
Structure of CBM3b of the major cellulosomal scaffoldin subunit ScaA from Acetivibrio cellulolyticus.
Yaniv O, Halfon Y, Shimon LJ, Bayer EA, Lamed R, Frolow F
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 8-13 (2012)
PMID: 22232162

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193225 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 16.79 KDa
Source organism: Acetivibrio cellulolyticus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9RPL0 (Residues: 973-1121; Coverage: 8%)
Gene name: cipV
Sequence domains: Cellulose binding domain
Structure domains: Endoglucanase-like

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand EDO 1 x EDO
Ligand NI 1 x NI
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6122
Unit cell:
a: 52.697Å b: 52.697Å c: 194.159Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.125 0.125 0.136
Expression system: Escherichia coli BL21

Quick links

Citations

4 citation in other articles

Are cellulosome scaffolding protein CipC and CBM3-containing protein HycP, involved in adherence of Clostridium cellulolyticum to cellulose?
Ferdinand et al. (2013)
3 more

3 mentions without citation

Structure of CBM3b of the major cellulosomal scaffoldin subunit ScaA from Acetivibrio cellulolyticus.
Yaniv et al. (2012)
2 more