PDBe 3zqc

X-ray diffraction
2.9Å resolution

Structure of the Trichomonas vaginalis Myb3 DNA-binding domain bound to a promoter sequence reveals a unique C-terminal beta-hairpin conformation

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Myb-like DNA-binding domain containing protein Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 131 amino acids
Theoretical weight: 15.38 KDa
Source organism: Trichomonas vaginalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A2D9X4 (Residues: 53-180; Coverage: 51%)
Gene name: TVAG_475500
Sequence domains: Myb-like DNA-binding domain
Structure domains: Homeodomain-like
MRE-1 Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Trichomonas vaginalis
Expression system: Not provided
MRE-1 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Trichomonas vaginalis
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P1
Unit cell:
a: 45.81Å b: 71.77Å c: 87.82Å
α: 94.68° β: 97.84° γ: 99.28°
R-values:
R R work R free
0.218 0.218 0.273
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided