› 3zlb

X-ray diffraction
1.78Å resolution

Crystal structure of phosphoglycerate kinase from Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb3zlb/pdb
PDB entry 3zlb coloured by chain, front view.
PDB entry 3zlb coloured by chain, side view.
PDB entry 3zlb coloured by chain, top view.
Source organism: Streptococcus pneumoniae D39
Primary publication:
Pneumococcal phosphoglycerate kinase interacts with plasminogen and its tissue activator.
Fulde M, Bernardo-García N, Rohde M, Nachtigall N, Frank R, Preissner KT, Klett J, Morreale A, Chhatwal GS, Hermoso JA, Bergmann S
Thromb Haemost 111 401-16 (2014)
PMID: 24196407

Function and Biology Details

Reaction catalysed: 
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170111 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoglycerate kinase Chain: A
Molecule details ›
Chain: A
Length: 398 amino acids
Theoretical weight: 41.99 KDa
Source organism: Streptococcus pneumoniae D39
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04LZ5 (Residues: 1-398; Coverage: 100%)
Gene names: SPD_0445, pgk
Sequence domains: Phosphoglycerate kinase
Structure domains: Phosphoglycerate kinase, N-terminal domain

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand ANP 1 x ANP
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 40.347Å b: 78.227Å c: 59.033Å
α: 90° β: 96.34° γ: 90°
R-values:
R R work R free
0.148 0.145 0.192
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Protein moonlighting: what is it, and why is it important?
Jeffery CJ. (2018)
4 more

1 mention without citation

Phosphoglycerate kinase: structural aspects and functions, with special emphasis on the enzyme from Kinetoplastea.
Rojas-Pirela et al. (2020)