› 3zjm

X-ray diffraction
1.5Å resolution

Ile(149)G11Phe mutation of M.acetivorans protoglobin in complex with cyanide

Released:
DOI: 10.2210/pdb3zjm/pdb
PDB entry 3zjm coloured by chain, front view.
PDB entry 3zjm coloured by chain, side view.
PDB entry 3zjm coloured by chain, top view.
Source organism: Methanosarcina acetivorans
Primary publication:
Structure and haem-distal site plasticity in Methanosarcina acetivorans protoglobin.
Pesce A, Tilleman L, Donné J, Aste E, Ascenzi P, Ciaccio C, Coletta M, Moens L, Viappiani C, Dewilde S, Bolognesi M, Nardini M
PLoS One 8 e66144 (2013)
PMID: 23776624

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin-sensor domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 195 amino acids
Theoretical weight: 23.04 KDa
Source organism: Methanosarcina acetivorans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TLY9 (Residues: 1-195; Coverage: 100%)
Gene name: MA_2883
Sequence domains: Protoglobin
Structure domains: Globins

Ligands and Environments

4 bound ligands:
Ligand HEM 3 x HEM
Ligand CYN 3 x CYN
Ligand GOL 3 x GOL
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 124.72Å b: 48.03Å c: 98.68Å
α: 90° β: 94.18° γ: 90°
R-values:
R R work R free
0.18 0.178 0.222
Expression system: Escherichia coli

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Citations

1 review citation

Enzymatic Mechanisms Involved in Evasion of Fungi to the Oxidative Stress: Focus on Scedosporium apiospermum.
Staerck et al. (2018)
8 other articles

2 mentions without citation

Structure and haem-distal site plasticity in Methanosarcina acetivorans protoglobin.
Pesce et al. (2013)
1 more