PDBe 3zj7

X-ray diffraction
2.5Å resolution

Crystal structure of strictosidine glucosidase in complex with inhibitor-1

Released:

Function and Biology Details

Reaction catalysed:
Strictosidine + H(2)O = D-glucose + strictosidine aglycone. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strictosidine-O-beta-D-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 532 amino acids
Theoretical weight: 60.96 KDa
Source organism: Rauvolfia serpentina
Expression system: Escherichia coli M15
UniProt:
  • Canonical: Q8GU20 (Residues: 1-532; Coverage: 100%)
Gene name: SGR1
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P4212
Unit cell:
a: 159.219Å b: 159.219Å c: 110.974Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.258 0.276
Expression system: Escherichia coli M15