› 3zj5

X-ray diffraction
1.95Å resolution

NEUROSPORA CRASSA CATALASE-3 EXPRESSED IN E. COLI, ORTHORHOMBIC FORM.

Released:
DOI: 10.2210/pdb3zj5/pdb
PDB entry 3zj5 coloured by chain, front view.
PDB entry 3zj5 coloured by chain, side view.
PDB entry 3zj5 coloured by chain, top view.
Source organism: Neurospora crassa
Primary publication:
Conformational stability and crystal packing: polymorphism in Neurospora crassa CAT-3.
Zárate-Romero A, Stojanoff V, Rojas-Trejo SP, Hansberg W, Rudiño-Piñera E
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 753-8 (2013)
PMID: 23832201

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase-3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 746 amino acids
Theoretical weight: 82.55 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9C169 (Residues: 1-719; Coverage: 100%)
Gene names: NCU00355, cat-3
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
4 bound ligands:
Ligand EDO 11 x EDO
Ligand PG4 12 x PG4
Ligand AE3 3 x AE3
Ligand TAR 1 x TAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 131.52Å b: 154.8Å c: 160.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.197
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Monofunctional Heme-Catalases.
Hansberg W. (2022)
1 other article

1 mention without citation

Conformational stability and crystal packing: polymorphism in Neurospora crassa CAT-3.
Zárate-Romero et al. (2013)