› 3zid

X-ray diffraction
2Å resolution

CetZ from Methanosaeta thermophila strain DSM 6194

Released:
DOI: 10.2210/pdb3zid/pdb
PDB entry 3zid coloured by chain, front view.
PDB entry 3zid coloured by chain, side view.
PDB entry 3zid coloured by chain, top view.
Source organism: Methanothrix thermoacetophila
Primary publication:
CetZ tubulin-like proteins control archaeal cell shape.
Duggin IG, Aylett CH, Walsh JC, Michie KA, Wang Q, Turnbull L, Dawson EM, Harry EJ, Whitchurch CB, Amos LA, Löwe J
Nature 519 362-5 (2015)
PMID: 25533961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106060 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tubulin-like protein CetZ Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 42.14 KDa
Source organism: Methanothrix thermoacetophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0B5R2 (Residues: 1-367; Coverage: 100%)
Gene names: Mthe_0239, cetZ
Sequence domains: Tubulin/FtsZ family, GTPase domain
Structure domains: Tubulin/FtsZ, GTPase domain

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 44.547Å b: 107.011Å c: 76.966Å
α: 90° β: 91.42° γ: 90°
R-values:
R R work R free
0.184 0.182 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Staying in Shape: the Impact of Cell Shape on Bacterial Survival in Diverse Environments.
Yang et al. (2016)
10 more

2 mentions without citation

CetZ tubulin-like proteins control archaeal cell shape.
Duggin et al. (2015)
1 more