PDBe 3zfs

Electron Microscopy
4Å resolution

Cryo-EM structure of the F420-reducing NiFe-hydrogenase from a methanogenic archaeon with bound substrate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
F420-reducing hydrogenase, subunit beta Chain: C
Molecule details ›
Chain: C
Length: 281 amino acids
Theoretical weight: 30.78 KDa
Source organism: Methanothermobacter marburgensis
UniProt:
  • Canonical: D9PYF6 (Residues: 1-281; Coverage: 100%)
Gene names: MTBMA_c16830, frhB
Sequence domains:
F420-reducing hydrogenase, subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 405 amino acids
Theoretical weight: 44.87 KDa
Source organism: Methanothermobacter marburgensis
UniProt:
  • Canonical: D9PYF9 (Residues: 1-405; Coverage: 100%)
Gene names: MTBMA_c16860, frhA
Sequence domains: Nickel-dependent hydrogenase
F420-reducing hydrogenase, subunit gamma Chain: B
Molecule details ›
Chain: B
Length: 275 amino acids
Theoretical weight: 30.27 KDa
Source organism: Methanothermobacter marburgensis
UniProt:
  • Canonical: D9PYF7 (Residues: 1-275; Coverage: 100%)
Gene names: MTBMA_c16840, frhG
Sequence domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-2097