› 3zd8

X-ray diffraction
2Å resolution

Potassium bound structure of E. coli ExoIX in P1

Released:
DOI: 10.2210/pdb3zd8/pdb
PDB entry 3zd8 coloured by chain, front view.
PDB entry 3zd8 coloured by chain, side view.
PDB entry 3zd8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of Escherichia coli ExoIX--implications for DNA binding and catalysis in flap endonucleases.
Anstey-Gilbert CS, Hemsworth GR, Flemming CS, Hodskinson MR, Zhang J, Sedelnikova SE, Stillman TJ, Sayers JR, Artymiuk PJ
Nucleic Acids Res 41 8357-67 (2013)
PMID: 23821668

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flap endonuclease Xni Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 28.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8X6R9 (Residues: 1-251; Coverage: 100%)
Gene names: ECs3658, Z4115, xni, ygdG
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P1
Unit cell:
a: 43.37Å b: 56.554Å c: 60.489Å
α: 110.35° β: 95.26° γ: 94.88°
R-values:
R R work R free
0.163 0.16 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Direct observation of DNA threading in flap endonuclease complexes.
AlMalki et al. (2016)
4 more

2 mentions without citation

Comprehensive classification of the PIN domain-like superfamily.
Matelska et al. (2017)
1 more