› 3zcj

X-ray diffraction
3.25Å resolution

Crystal structure of Helicobacter pylori T4SS protein CagL in a tetragonal crystal form with a helical RGD-motif (6 Mol per ASU)

Released:
DOI: 10.2210/pdb3zcj/pdb
PDB entry 3zcj coloured by chain, front view.
PDB entry 3zcj coloured by chain, side view.
PDB entry 3zcj coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
A helical RGD motif promoting cell adhesion: crystal structures of the Helicobacter pylori type IV secretion system pilus protein CagL.
Barden S, Lange S, Tegtmeyer N, Conradi J, Sewald N, Backert S, Niemann HH
Structure 21 1931-41 (2013)
PMID: 24076404

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127738 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cag pathogenicity island protein (Cag18) Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 220 amino acids
Theoretical weight: 25.48 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O25272 (Residues: 21-237; Coverage: 92%)
Gene name: HP_0539
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

4 bound ligands:
Ligand PEG 1 x PEG
Ligand GOL 8 x GOL
Ligand PO4 2 x PO4
Ligand K 1 x K
1 modified residue:
Ligand MLY 120 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P43212
Unit cell:
a: 197.122Å b: 197.122Å c: 106.808Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Type IV secretion in Gram-negative and Gram-positive bacteria.
Grohmann et al. (2018)
14 more

5 mentions without citation

Helicobacter pylori CagL Y58/E59 mutation turns-off type IV secretion-dependent delivery of CagA into host cells.
Tegtmeyer et al. (2014)
4 more