› 3x17

X-ray diffraction
2.15Å resolution

Crystal structure of metagenome-derived glycoside hydrolase family 9 endoglucanase

Released:
DOI: 10.2210/pdb3x17/pdb
PDB entry 3x17 coloured by chain, front view.
PDB entry 3x17 coloured by chain, side view.
PDB entry 3x17 coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Structure, activity, and stability of metagenome-derived glycoside hydrolase family 9 endoglucanase with an N-terminal Ig-like domain.
Okano H, Kanaya E, Ozaki M, Angkawidjaja C, Kanaya S
Protein Sci 24 408-19 (2015)
PMID: 25545469

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 559 amino acids
Theoretical weight: 61.11 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: W8PF21 (Residues: 20-577; Coverage: 97%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 84.631Å b: 89.913Å c: 151.157Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.246
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Metagenomics: novel enzymes from non-culturable microbes.
Berini et al. (2017)
5 other articles

7 mentions without citation

Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes.
Romero Victorica et al. (2020)
6 more