› 3wwh

X-ray diffraction
1.65Å resolution

Crystal structure of the first R-stereoselective -transaminase identified from Arthrobacter sp. KNK168 (FERM-BP-5228)

Released:
DOI: 10.2210/pdb3wwh/pdb
PDB entry 3wwh coloured by chain, front view.
PDB entry 3wwh coloured by chain, side view.
PDB entry 3wwh coloured by chain, top view.
Source organism: Arthrobacter sp. KNK168
Primary publication:
A new target region for changing the substrate specificity of amine transaminases.
Guan LJ, Ohtsuka J, Okai M, Miyakawa T, Mase T, Zhi Y, Hou F, Ito N, Iwasaki A, Yasohara Y, Tanokura M
Sci Rep 5 10753 (2015)
PMID: 26030619

Function and Biology Details

Reaction catalysed: 
L-alanine + 3-oxopropanoate = pyruvate + beta-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123675 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(R)-amine transaminase Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 36.45 KDa
Source organism: Arthrobacter sp. KNK168
Expression system: Escherichia coli
UniProt:
  • Canonical: F7J696 (Residues: 1-330; Coverage: 100%)
Gene name: TAS
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P42212
Unit cell:
a: 80.62Å b: 80.62Å c: 93.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.172
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Enzymes useful for chiral compound synthesis: structural biology, directed evolution, and protein engineering for industrial use.
Kataoka et al. (2016)
1 more

2 mentions without citation

Structural Consideration of the Working Mechanism of Fold Type I Transaminases From Eubacteria: Overt and Covert Movement.
Kwon et al. (2019)
1 more