› 3wn2

X-ray diffraction
2.1Å resolution

Crystal Structure of Streptomyces coelicolor alpha-L-arabinofuranosidase in complex with xylohexaose

Released:
DOI: 10.2210/pdb3wn2/pdb
PDB entry 3wn2 coloured by chain, front view.
PDB entry 3wn2 coloured by chain, side view.
PDB entry 3wn2 coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
Crystal structure and characterization of the glycoside hydrolase family 62 α-L-arabinofuranosidase from Streptomyces coelicolor.
Maehara T, Fujimoto Z, Ichinose H, Michikawa M, Harazono K, Kaneko S
J Biol Chem 289 7962-72 (2014)
PMID: 24482228

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Extracellular exo-alpha-L-arabinofuranosidase Chain: A
Molecule details ›
Chain: A
Length: 438 amino acids
Theoretical weight: 46.52 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Streptomyces lividans
UniProt:
  • Canonical: O54161 (Residues: 38-475; Coverage: 100%)
Gene names: SC7H1.02, SCO5932, abfB
Sequence domains:
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
4 bound ligands:
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand TRS 1 x TRS
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P41212
Unit cell:
a: 97.437Å b: 97.437Å c: 103.242Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.191
Expression system: Streptomyces lividans

Quick links

Citations

1 review citation

GH62 arabinofuranosidases: Structure, function and applications.
Wilkens et al. (2017)
15 other articles

5 mentions without citation

Crystal structure and characterization of the glycoside hydrolase family 62 α-L-arabinofuranosidase from Streptomyces coelicolor.
Maehara et al. (2014)
4 more