PDBe 3wd5

X-ray diffraction
3.1Å resolution

Crystal structure of TNFalpha in complex with Adalimumab Fab fragment

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tumor necrosis factor, soluble form Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 17.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01375 (Residues: 77-233; Coverage: 67%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
Adalimumab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.27 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Adalimumab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.33 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: I213
Unit cell:
a: 161.849Å b: 161.849Å c: 161.849Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.187 0.275
Expression systems:
  • Escherichia coli
  • Cricetulus griseus