› 3w8w

X-ray diffraction
1.95Å resolution

The crystal structure of EncM

Released:
DOI: 10.2210/pdb3w8w/pdb
PDB entry 3w8w coloured by chain, front view.
PDB entry 3w8w coloured by chain, side view.
PDB entry 3w8w coloured by chain, top view.
Source organism: Streptomyces maritimus
Primary publication:
Flavin-mediated dual oxidation controls an enzymatic Favorskii-type rearrangement.
Teufel R, Miyanaga A, Michaudel Q, Stull F, Louie G, Noel JP, Baran PS, Palfey B, Moore BS
Nature 503 552-556 (2013)
PMID: 24162851

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding PCMH-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 50.41 KDa
Source organism: Streptomyces maritimus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KHK2 (Residues: 1-464; Coverage: 100%)
Gene name: encM
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 71.408Å b: 85.278Å c: 79.669Å
α: 90° β: 99.84° γ: 90°
R-values:
R R work R free
0.193 0.191 0.243
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Oxidative Cyclization in Natural Product Biosynthesis.
Tang et al. (2017)
17 more

4 mentions without citation

N5 Is the New C4a: Biochemical Functionalization of Reduced Flavins at the N5 Position.
Beaupre et al. (2020)
3 more