› 3w2h

X-ray diffraction
1.75Å resolution

Crystal structure of oxidation intermediate (1min) of NADH-cytochrome b5 reductase from pig liver

Released:
DOI: 10.2210/pdb3w2h/pdb
PDB entry 3w2h coloured by chain, front view.
PDB entry 3w2h coloured by chain, side view.
PDB entry 3w2h coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Elucidations of the catalytic cycle of NADH-cytochrome b5 reductase by X-ray crystallography: new insights into regulation of efficient electron transfer.
Yamada M, Tamada T, Takeda K, Matsumoto F, Ohno H, Kosugi M, Takaba K, Shoyama Y, Kimura S, Kuroki R, Miki K
J Mol Biol 425 4295-306 (2013)
PMID: 23831226

Function and Biology Details

Reaction catalysed: 
NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160717 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH-cytochrome b5 reductase 3 Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 30.79 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P83686 (Residues: 2-272; Coverage: 100%)
Gene names: CYB5R3, DIA1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 57.746Å b: 72.873Å c: 85.713Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.183
Expression system: Escherichia coli

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Citations

2 review citations

Cytochrome b5 reductases: Redox regulators of cell homeostasis.
Hall et al. (2022)
1 more