PDBe 3w1k

X-ray diffraction
7.5Å resolution

Crystal structure of the selenocysteine synthase SelA and tRNASec complex

Released:

Function and Biology Details

Reaction catalysed:
L-seryl-tRNA(Sec) + selenophosphate = L-selenocysteinyl-tRNA(Sec) + phosphate. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 20-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
L-seryl-tRNA(Sec) selenium transferase Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 452 amino acids
Theoretical weight: 50.93 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67140 (Residues: 1-452; Coverage: 100%)
Gene names: aq_1031, selA
Sequence domains: L-seryl-tRNA selenium transferase
selenocysteine tRNA Chains: F, G, H, I, J
Molecule details ›
Chains: F, G, H, I, J
Length: 95 nucleotides
Theoretical weight: 30.49 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Not provided

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 148.553Å b: 355.972Å c: 165.495Å
α: 90° β: 115.41° γ: 90°
R-values:
R R work R free
0.194 0.194 0.24
Expression systems:
  • Escherichia coli
  • Not provided