PDBe 3w0w

X-ray diffraction
2.6Å resolution

The complex between T36-5 TCR and HLA-A24 bound to HIV-1 Nef134-10(2F) peptide in space group P212121

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T36-5 TCR beta chain Chain: E
Molecule details ›
Chain: E
Length: 242 amino acids
Theoretical weight: 27.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
T36-5 TCR alpha chain Chain: D
Molecule details ›
Chain: D
Length: 205 amino acids
Theoretical weight: 22.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Protein Nef Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.27 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: Q9YYU3 (Residues: 143-152; Coverage: 5%)
Gene name: nef
HLA class I histocompatibility antigen, A-24 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05534 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 50.921Å b: 86.441Å c: 251.772Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.25
Expression systems:
  • Escherichia coli
  • Not provided