EC 126.96.36.199: Pyrrolysine--tRNA(Pyl) ligase
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl- tRNA(Pyl).
- In organisms such as Methanosarcina barkeri that incorporate the modified amino acid pyrrolysine (Pyl) into certain methylamine methyltransferases, an unusual tRNA(Pyl), with a CUA anticodon, can be charged directly with pyrrolysine by this class II aminoacyl- -tRNA ligase.
- Specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids.
L-pyrrolysine:tRNA(Pyl) ligase (AMP-forming)
- Pyrrolysyl-tRNA synthetase
Protein family (Pfam)
Domain description: tRNA synthetases class II core domain (F)
Domain description: Phenylalanyl-tRNA synthetase
Domain description: Aminoacyl-tRNA synthetase, class II
Domain description: Pyrrolysyl-tRNA ligase, C-terminal
Domain description: Rossmann-like alpha/beta/alpha sandwich fold
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Helix Hairpins
Homology: Helix hairpin binOccurring in:
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: BirA Bifunctional Protein; domain 2
Homology: Bira Bifunctional Protein; Domain 2Occurring in: