Structure analysis

Crystal structure of the catalytic domain of pyrrolysyl-tRNA synthetase in triclinic crystal form

X-ray diffraction
2.3Å resolution
Source organism: Methanosarcina mazei
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

Assemblies

Assembly 1
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Multimeric state: homo dimer

Binding statistics and energies are not available for this assembly
Assembly 2 (preferred)
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Multimeric state: homo dimer
Accessible surface area: 23200 Å2
Buried surface area: 7200 Å2
Dissociation area: 2,400 Å2
Dissociation energy (ΔGdiss): 31 kcal/mol
Dissociation entropy (TΔSdiss): 14 kcal/mol
Interface energy (ΔGint): -42 kcal/mol
Symmetry number: 2

Macromolecules

Chains: A, B, C, D
Length: 291 amino acids
Theoretical weight: 33.34 KDa
Source organism: Methanosarcina mazei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8PWY1 (Residues: 184-454; Coverage: 60%)
Gene names: MM_1445, pylS
Pfam: tRNA synthetases class II core domain (F)
InterPro:
CATH:

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