PDBe 3vqx

X-ray diffraction
2.3Å resolution

Crystal structure of the catalytic domain of pyrrolysyl-tRNA synthetase in triclinic crystal form

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl- tRNA(Pyl). 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrrolysine--tRNA ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 291 amino acids
Theoretical weight: 33.34 KDa
Source organism: Methanosarcina mazei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8PWY1 (Residues: 184-454; Coverage: 60%)
Gene names: MM_1445, pylS
Sequence domains: tRNA synthetases class II core domain (F)
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P1
Unit cell:
a: 62.798Å b: 69.422Å c: 83.326Å
α: 106.92° β: 91.41° γ: 113.83°
R-values:
R R work R free
0.212 0.212 0.27
Expression system: Escherichia coli