PDBe 3vht

X-ray diffraction
2.4Å resolution

Crystal structure of GFP-Wrnip1 UBZ domain fusion protein in complex with ubiquitin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin-60S ribosomal protein L40 Chain: C
Molecule details ›
Chain: C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: E9Q9J0 (Residues: 1-76; Coverage: 79%)
Gene name: Uba52
Sequence domains: Ubiquitin family
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.31 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-230; Coverage: 97%)
Gene name: GFP
Sequence domains: Green fluorescent protein
ATPase WRNIP1 Chain: B
Molecule details ›
Chain: B
Length: 271 amino acids
Theoretical weight: 30.54 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96S55 (Residues: 9-30, 31-46; Coverage: 6%)
Gene names: WHIP, WRNIP1

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 88.95Å b: 143.692Å c: 106.864Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.238
Expression system: Escherichia coli