PDBe 3vgl

X-ray diffraction
1.55Å resolution

Crystal structure of a ROK family glucokinase from Streptomyces griseus in complex with glucose and AMPPNP

Released:

Function and Biology Details

Reaction catalysed:
ATP + D-glucose = ADP + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucokinase Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 33.44 KDa
Source organism: Streptomyces griseus subsp. griseus NBRC 13350
Expression system: Escherichia coli
UniProt:
  • Canonical: B1VZT1 (Residues: 1-313; Coverage: 100%)
Gene names: SGR_5377, glkA
Sequence domains: ROK family
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: I222
Unit cell:
a: 69.58Å b: 87.73Å c: 125.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.202
Expression system: Escherichia coli