PDBe 3vbg

X-ray diffraction
2.8Å resolution

Structure of hDM2 with Dimer Inducing Indolyl Hydantoin RO-2443

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Mdm2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 85 amino acids
Theoretical weight: 10.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q00987 (Residues: 25-109; Coverage: 17%)
  • Best match: Q00987-8 (Residues: 1-48)
Gene name: MDM2
Sequence domains: SWIB/MDM2 domain
Structure domains: SWIB/MDM2 domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P32
Unit cell:
a: 64.392Å b: 64.392Å c: 167.587Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.22
Expression system: Escherichia coli BL21