› 3uxu

X-ray diffraction
2.71Å resolution

The structure of the catalytic domain of the Sulfolobus Spindle-shaped viral integrase reveals an evolutionarily conserved catalytic core and supports a mechanism of DNA cleavage in trans

Released:
DOI: 10.2210/pdb3uxu/pdb
PDB entry 3uxu coloured by chain, front view.
PDB entry 3uxu coloured by chain, side view.
PDB entry 3uxu coloured by chain, top view.
Source organism: Sulfolobus spindle-shaped virus 1
Primary publication:
The structure of an archaeal viral integrase reveals an evolutionarily conserved catalytic core yet supports a mechanism of DNA cleavage in trans.
Eilers BJ, Young MJ, Lawrence CM
J Virol 86 8309-13 (2012)
PMID: 22593158

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrase Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 20.02 KDa
Source organism: Sulfolobus spindle-shaped virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20214 (Residues: 173-335; Coverage: 49%)
Gene name: d335
Sequence domains: Archaeal phage integrase
Structure domains: Intergrase catalytic core

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6522
Unit cell:
a: 73.963Å b: 73.963Å c: 176.251Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Archaeal extrachromosomal genetic elements.
Wang et al. (2015)
5 more

3 mentions without citation

A Review on Viral Metagenomics in Extreme Environments.
Dávila-Ramos et al. (2019)
2 more