› 3um1

X-ray diffraction
2.71Å resolution

Crystal structure of the Brox Bro1 domain in complex with the C-terminal tail of CHMP5

Released:
DOI: 10.2210/pdb3um1/pdb
PDB entry 3um1 coloured by chain, front view.
PDB entry 3um1 coloured by chain, side view.
PDB entry 3um1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Two distinct binding modes define the interaction of Brox with the C-terminal tails of CHMP5 and CHMP4B.
Mu R, Dussupt V, Jiang J, Sette P, Rudd V, Chuenchor W, Bello NF, Bouamr F, Xiao TS
Structure 20 887-98 (2012)
PMID: 22484091

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178446 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BRO1 domain-containing protein BROX Chains: A, D
Molecule details ›
Chains: A, D
Length: 376 amino acids
Theoretical weight: 42.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5VW32 (Residues: 2-377; Coverage: 92%)
Gene names: BROFTI, BROX, C1orf58
Sequence domains: BRO1-like domain
Structure domains: alix/aip1 like domains
Charged multivesicular body protein 5 Chains: B, E
Molecule details ›
Chains: B, E
Length: 69 amino acids
Theoretical weight: 7.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZZ3 (Residues: 151-219; Coverage: 32%)
Gene names: C9orf83, CGI-34, CHMP5, HSPC177, PNAS-114, PNAS-2, SNF7DC2

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 68.92Å b: 111.39Å c: 116.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.186 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Membrane fission reactions of the mammalian ESCRT pathway.
McCullough et al. (2013)
3 more

1 mention without citation

Two distinct binding modes define the interaction of Brox with the C-terminal tails of CHMP5 and CHMP4B.
Mu et al. (2012)