› 3ucm

X-ray diffraction
2.51Å resolution

Coccomyxa beta-carbonic anhydrase in complex with thiocyanate

Released:
DOI: 10.2210/pdb3ucm/pdb
PDB entry 3ucm coloured by chain, front view.
PDB entry 3ucm coloured by chain, side view.
PDB entry 3ucm coloured by chain, top view.
Source organism: Coccomyxa sp. PA
Primary publication:
Structural studies of β-carbonic anhydrase from the green alga Coccomyxa: inhibitor complexes with anions and acetazolamide.
Huang S, Hainzl T, Grundström C, Forsman C, Samuelsson G, Sauer-Eriksson AE
PLoS One 6 e28458 (2011)
PMID: 22162771

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 24.73 KDa
Source organism: Coccomyxa sp. PA
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96554 (Residues: 1-227; Coverage: 100%)
Sequence domains: Carbonic anhydrase
Structure domains: Carbonic anhydrase

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SCN 3 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P43212
Unit cell:
a: 76.835Å b: 76.835Å c: 222.139Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.189 0.25
Expression system: Escherichia coli

Quick links

Citations

4 review citations

How many carbonic anhydrase inhibition mechanisms exist?
Supuran CT. (2016)
3 more

1 mention without citation

Structural studies of β-carbonic anhydrase from the green alga Coccomyxa: inhibitor complexes with anions and acetazolamide.
Huang et al. (2011)