› 3ub6

X-ray diffraction
1.38Å resolution

Periplasmic portion of the Helicobacter pylori chemoreceptor TlpB with urea bound

Released:
DOI: 10.2210/pdb3ub6/pdb
PDB entry 3ub6 coloured by chain, front view.
PDB entry 3ub6 coloured by chain, side view.
PDB entry 3ub6 coloured by chain, top view.
Source organism: Helicobacter pylori SS1
Primary publication:
Structure and proposed mechanism for the pH-sensing Helicobacter pylori chemoreceptor TlpB.
Goers Sweeney E, Henderson JN, Goers J, Wreden C, Hicks KG, Foster JK, Parthasarathy R, Remington SJ, Guillemin K
Structure 20 1177-88 (2012)
PMID: 22705207

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110346 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting chemotaxis transmembrane sensory protein (MCP-like protein) Chains: A, B
Molecule details ›
Chains: A, B
Length: 181 amino acids
Theoretical weight: 20.69 KDa
Source organism: Helicobacter pylori SS1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B6JPK4 (Residues: 30-208; Coverage: 32%)
Gene names: HPP12_0105, tlpB
Sequence domains: Single Cache domain 2
Structure domains: PAS domain

Ligands and Environments

4 bound ligands:
Ligand URE 2 x URE
Ligand SO4 3 x SO4
Ligand PEG 1 x PEG
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P212121
Unit cell:
a: 79.608Å b: 81.881Å c: 94.534Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.123 0.146
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

The effect of bacterial chemotaxis on host infection and pathogenicity.
Matilla et al. (2018)
13 more

7 mentions without citation

Sensory Repertoire of Bacterial Chemoreceptors.
Ortega et al. (2017)
6 more