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Guanylate Kinase Domains of the MAGUK Family Scaffold Proteins as Specific Phospho-Protein Binding Modules

X-ray diffraction
2.7Å resolution
PDB entry 3uat coloured by chain, front view.
PDB entry 3uat coloured by chain, side view.
PDB entry 3uat coloured by chain, top view.
Source organism: Rattus norvegicus
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: G-protein signaling modulator 2 and Disks large homolog 1
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Multimeric state: hetero dimer
Accessible surface area: 15464.88 Å2
Buried surface area: 1576.89 Å2
Dissociation area: 788.44 Å2
Dissociation energy (ΔGdiss): 7.26 kcal/mol
Dissociation entropy (TΔSdiss): 8.71 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-112598

Macromolecules

Disks large homolog 1
Chain: A
Length: 296 amino acids
Theoretical weight: 34.3 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62696 (Residues: 578-911; Coverage: 32%)
Gene names: Dlg1, Dlgh1
Pfam:
InterPro:
CATH:
Disks large homolog 1 in PDB entry 3uat, assembly 1, front view.
Disks large homolog 1 in PDB entry 3uat, assembly 1, side view.
Disks large homolog 1 in PDB entry 3uat, assembly 1, top view.
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G-protein signaling modulator 2
Chain: B
Length: 18 amino acids
Theoretical weight: 2.25 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: D3ZCE5 (Residues: 404-421; Coverage: 3%)
Gene name: Gpsm2
G-protein signaling modulator 2 in PDB entry 3uat, assembly 1, front view.
G-protein signaling modulator 2 in PDB entry 3uat, assembly 1, side view.
G-protein signaling modulator 2 in PDB entry 3uat, assembly 1, top view.
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