› 3u3o

X-ray diffraction
2Å resolution

Crystal structure of Human SULT1A1 bound to PAP and two 3-Cyano-7-hydroxycoumarin

Released:
DOI: 10.2210/pdb3u3o/pdb
PDB entry 3u3o coloured by chain, front view.
PDB entry 3u3o coloured by chain, side view.
PDB entry 3u3o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The molecular basis for the broad substrate specificity of human sulfotransferase 1A1.
Berger I, Guttman C, Amar D, Zarivach R, Aharoni A
PLoS One 6 e26794 (2011)
PMID: 22069470

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 1A1 Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 36.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50225 (Residues: 1-295; Coverage: 100%)
Gene names: OK/SW-cl.88, STP, STP1, SULT1A1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand 3QV 2 x 3QV
Ligand A3P 1 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 72.18Å b: 123.13Å c: 44.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

The Regulation of Steroid Action by Sulfation and Desulfation.
Mueller et al. (2015)
10 more

4 mentions without citation

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
3 more