› 3ty7

X-ray diffraction
2.4Å resolution

Crystal Structure of Aldehyde Dehydrogenase family Protein from Staphylococcus aureus

Released:
DOI: 10.2210/pdb3ty7/pdb
PDB entry 3ty7 coloured by chain, front view.
PDB entry 3ty7 coloured by chain, side view.
PDB entry 3ty7 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Entry authors: Kim Y, Joachimiak G, Jedrzejczak R, Rubin E, Ioerger T, Sacchettini J, Joachimiak A, Midwest Center for Structural Genomics (MCSG), Structures of Mtb Proteins Conferring Susceptibility to Known Mtb Inhibitors (MTBI)

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189393 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative aldehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 478 amino acids
Theoretical weight: 52.63 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99SD6 (Residues: 1-475; Coverage: 100%)
Gene name: SAV2122
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 7 x GOL
Ligand PEG 2 x PEG
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: R3
Unit cell:
a: 173.448Å b: 173.448Å c: 96.569Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.185 0.212
Expression system: Escherichia coli BL21

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